Publications
2011 |
Reichhart, Jean-Marc; Gubb, David; Leclerc, Vincent The Drosophila serpins: multiple functions in immunity and morphogenesis Article de journal Meth. Enzymol., 499 , p. 205–225, 2011, ISSN: 1557-7988. Résumé | Liens | BibTeX | Étiquettes: Animals, Immunity, Innate, M3i, Morphogenesis, reichhart, Serpins, Signal Transduction @article{reichhart_drosophila_2011, title = {The Drosophila serpins: multiple functions in immunity and morphogenesis}, author = {Jean-Marc Reichhart and David Gubb and Vincent Leclerc}, doi = {10.1016/B978-0-12-386471-0.00011-0}, issn = {1557-7988}, year = {2011}, date = {2011-01-01}, journal = {Meth. Enzymol.}, volume = {499}, pages = {205--225}, abstract = {Members of the serpin superfamily of proteins have been found in all living organisms, although rarely in bacteria or fungi. They have been extensively studied in mammals, where many rapid physiological responses are regulated by inhibitory serpins. In addition to the inhibitory serpins, a large group of noninhibitory proteins with a conserved serpin fold have also been identified in mammals. These noninhibitory proteins have a wide range of functions, from storage proteins to molecular chaperones, hormone transporters, and tumor suppressors. In contrast, until recently, very little was known about insect serpins in general, or Drosophila serpins in particular. In the last decade, however, there has been an increasing interest in the serpin biology of insects. It is becoming clear that, like in mammals, a similar wide range of physiological responses are regulated in insects and that noninhibitory serpin-fold proteins also play key roles in insect biology. Drosophila is also an important model organism that can be used to study human pathologies (among which serpinopathies or other protein conformational diseases) and mechanisms of regulation of proteolytic cascades in health or to develop strategies for control of insect pests and disease vectors. As most of our knowledge on insect serpins comes from studies on the Drosophila immune response, we survey here the Drosophila serpin literature and describe the laboratory techniques that have been developed to study serpin-regulated responses in this model genetic organism.}, keywords = {Animals, Immunity, Innate, M3i, Morphogenesis, reichhart, Serpins, Signal Transduction}, pubstate = {published}, tppubtype = {article} } Members of the serpin superfamily of proteins have been found in all living organisms, although rarely in bacteria or fungi. They have been extensively studied in mammals, where many rapid physiological responses are regulated by inhibitory serpins. In addition to the inhibitory serpins, a large group of noninhibitory proteins with a conserved serpin fold have also been identified in mammals. These noninhibitory proteins have a wide range of functions, from storage proteins to molecular chaperones, hormone transporters, and tumor suppressors. In contrast, until recently, very little was known about insect serpins in general, or Drosophila serpins in particular. In the last decade, however, there has been an increasing interest in the serpin biology of insects. It is becoming clear that, like in mammals, a similar wide range of physiological responses are regulated in insects and that noninhibitory serpin-fold proteins also play key roles in insect biology. Drosophila is also an important model organism that can be used to study human pathologies (among which serpinopathies or other protein conformational diseases) and mechanisms of regulation of proteolytic cascades in health or to develop strategies for control of insect pests and disease vectors. As most of our knowledge on insect serpins comes from studies on the Drosophila immune response, we survey here the Drosophila serpin literature and describe the laboratory techniques that have been developed to study serpin-regulated responses in this model genetic organism. |
2007 |
Gubb, David; Robertson, Andrew S; Troxler, Laurent; Reichhart, Jean-Marc Drosophila Serpins: Regulatory Cascades in Innate Immunity and Morphogenesis incollection Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity, p. 207–227, Silverman GA and Lomas DA, London UK, 2007. BibTeX | Étiquettes: bioinformatic, innate immunity, M3i, Morphogenesis, regulatory Cascades, reichhart, Serpins @incollection{gubb_drosophila_2007, title = {Drosophila Serpins: Regulatory Cascades in Innate Immunity and Morphogenesis}, author = {David Gubb and Andrew S Robertson and Laurent Troxler and Jean-Marc Reichhart}, year = {2007}, date = {2007-01-01}, booktitle = {Molecular and Cellular Aspects of the Serpinopathies and Disorders in Serpin Activity}, pages = {207--227}, publisher = {Silverman GA and Lomas DA}, address = {London UK}, edition = {World Scientific Pub.}, keywords = {bioinformatic, innate immunity, M3i, Morphogenesis, regulatory Cascades, reichhart, Serpins}, pubstate = {published}, tppubtype = {incollection} } |
2003 |
Kambris, Zakaria; Bilak, Hana; D'Alessandro, Rosalba; Belvin, Marcia; Imler, Jean-Luc; Capovilla, Maria DmMyD88 controls dorsoventral patterning of the Drosophila embryo Article de journal EMBO reports, 4 (1), p. 64–69, 2003, ISSN: 1469-221X. Résumé | Liens | BibTeX | Étiquettes: Adaptor Proteins, Alleles, Animals, Antigens, Base Sequence, Cell Surface, Complementary, Developmental, Differentiation, DNA, DNA Transposable Elements, Egg Proteins, Embryo, Exons, Female, Gene Expression Regulation, Genetically Modified, Genotype, imler, Immunity, Immunologic, Innate, Insertional, M3i, Male, messenger, Morphogenesis, Mutagenesis, Myeloid Differentiation Factor 88, Nonmammalian, Oocytes, Protein Biosynthesis, Protein Structure, Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Signal Transducing, Tertiary, Toll-Like Receptors, Zygote @article{kambris_dmmyd88_2003, title = {DmMyD88 controls dorsoventral patterning of the Drosophila embryo}, author = {Zakaria Kambris and Hana Bilak and Rosalba D'Alessandro and Marcia Belvin and Jean-Luc Imler and Maria Capovilla}, doi = {10.1038/sj.embor.embor714}, issn = {1469-221X}, year = {2003}, date = {2003-01-01}, journal = {EMBO reports}, volume = {4}, number = {1}, pages = {64--69}, abstract = {MyD88 is an adapter protein in the signal transduction pathway mediated by interleukin-1 (IL-1) and Toll-like receptors. A Drosophila homologue of MyD88 (DmMyD88) was recently shown to be required for the Toll-mediated immune response. In Drosophila, the Toll pathway was originally characterized for its role in the dorsoventral patterning of the embryo. We found that, like Toll, DmMyD88 messenger RNA is maternally supplied to the embryo. Here we report the identification of a new mutant allele of DmMyD88, which generates a protein lacking the carboxy-terminal extension, normally located downstream of the Toll/IL-1 receptor domain. Homozygous mutant female flies lay dorsalized embryos that are rescued by expression of a transgenic DmMyD88 complementary DNA. The DmMyD88 mutation blocks the ventralizing activity of a gain-of-function Toll mutation. These results show that DmMyD88 encodes an essential component of the Toll pathway in dorsoventral pattern formation.}, keywords = {Adaptor Proteins, Alleles, Animals, Antigens, Base Sequence, Cell Surface, Complementary, Developmental, Differentiation, DNA, DNA Transposable Elements, Egg Proteins, Embryo, Exons, Female, Gene Expression Regulation, Genetically Modified, Genotype, imler, Immunity, Immunologic, Innate, Insertional, M3i, Male, messenger, Morphogenesis, Mutagenesis, Myeloid Differentiation Factor 88, Nonmammalian, Oocytes, Protein Biosynthesis, Protein Structure, Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Signal Transducing, Tertiary, Toll-Like Receptors, Zygote}, pubstate = {published}, tppubtype = {article} } MyD88 is an adapter protein in the signal transduction pathway mediated by interleukin-1 (IL-1) and Toll-like receptors. A Drosophila homologue of MyD88 (DmMyD88) was recently shown to be required for the Toll-mediated immune response. In Drosophila, the Toll pathway was originally characterized for its role in the dorsoventral patterning of the embryo. We found that, like Toll, DmMyD88 messenger RNA is maternally supplied to the embryo. Here we report the identification of a new mutant allele of DmMyD88, which generates a protein lacking the carboxy-terminal extension, normally located downstream of the Toll/IL-1 receptor domain. Homozygous mutant female flies lay dorsalized embryos that are rescued by expression of a transgenic DmMyD88 complementary DNA. The DmMyD88 mutation blocks the ventralizing activity of a gain-of-function Toll mutation. These results show that DmMyD88 encodes an essential component of the Toll pathway in dorsoventral pattern formation. |