M3i

@article{,
title = {A fast selenium derivatization strategy for crystallization and phasing of RNA structures},
author = {V Olieric and U Rieder and K Lang and A Serganov and C Schulze-Briese and R Micura and P Dumas and E Ennifar},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19228585},
isbn = {19228585},
year = {2009},
date = {2009-01-01},
journal = {RNA},
volume = {15},
number = {4},
pages = {707-715},
abstract = {Site-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality.},
note = {1469-9001 (Electronic)
Journal Article
Research Support, Non-U.S. Gov't
Validation Studies},
keywords = {DUMAS Base Sequence Crystallography, ENNIFAR, Unité ARN, X-Ray/*methods HIV-1/chemistry/genetics Nucleic Acid Conformation RNA/*chemistry Selenium/*metabolism Staining and Labeling/*methods},
pubstate = {published},
tppubtype = {article}
}